A liposome-PCR assay for the ultrasensitive detection of biological toxins
Date: 20.6.2006
We describe an ultrasensitive immunoassay for detecting biotoxins that uses liposomes with encapsulated DNA reporters, and ganglioside receptors embedded in the bilayer, as a detection reagent. After immobilization of the target biotoxin by a capture antibody and co-binding of the detection reagent, the liposomes are ruptured to release the reporters, which are quantified by real-time PCR.
Assays for cholera and botulinum toxins are several orders of magnitude more sensitive than current detection methods.
"Source":[ http://www.nature.com/nbt/journal/v24/n5/abs/nbt1201.html]
