A suggestion of enzymatic biosensor for determination of chlorinated pesticides
Date: 9.10.2006
Introduction. Chlorinated pesticides such as derivates of DTT, hexachlorobenzene and many others have been used to protect of plants. It is a common knowledge that residues of these pesticides deposit in adipose tissues at animals and could be transferred from here to eggs or to breast milk of mammals. A higher concentration of these compounds could be responsible for infertility and for tumour disease. Therefore a development of simple and accurate tool for detection of them is need.
Objective. The aim of this work was to suggest a biosensor used for determination of chlorinated pesticides. Materials and methods. Stationary electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was carbon paste electrode. A flow injection analysis coupled with electrochemical detector consisted of solvent delivery pump, a guard cell, a reaction coil (1 m) and an electrochemical detector. The electrochemical detector includes one low volume flow-through analytical cells, which is consisted of glassy carbon working electrode, palladium electrode as reference electrode and auxiliary carbon electrode, and Coulochem III as a control module.
Results. Our suggested biosensor is based on a determination of chlorides on carbon paste and/or glassy carbon electrode both in stationary and flow system. We improved sensitivity and selectivity of the analysis by stationary electrochemical system, particularly by carbon past electrode, through modification of the electrode by silver nitrate. We were able to determine chlorides in units of 10-8 M due to this modification. After that we evaluated that we were able to determine chloride, we suggested our biosensor, which were consisted from the electrode and a dehalogenases. The enzymes were capable of dechlorinating chlorinated hydrocarbons. We applied the biosensor to detect a 1-chlorohexane and were successful. Moreover, we attempted to determine chlorinated hydrocarbons by flow technique with electrochemical detection.
Conclusions. Commonly used methods for determination of chlorinated pesticides are time consuming and highly cost. Here, we suggested a technique for simple and low cost determination of these compounds.
MIKELOVÁ, Radka - PROKOP, Zbyněk - HRADECKÝ, Jan - TRNKOVÁ, Libuše - ADAM, Vojtěch - DAMBORSKÝ, Jiří - KIZEK, René. A suggestion of enzymatic biosensor for determination of chlorinated pesticides. In 11th International conference on electroanalysis. Vyd. první. Bordeaux : European Society for ElectroAnalytical Chemistry, 2006. pp. P1-045. 11.6.2006, Bordeaux.
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