Animal Cell Electroporation and Electrofusion Protocols, Vol. 48

This book provides well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells. The collection is distinguished by its coverage of important model cell types from many organisms and tissue types, including Chinese hamster ovary cells, normal human fibroblasts, and human lymphoblastoid cells. It also includes detailed instructions for the growth and preparation of specific cells to achieve optimum animal cell transfection and proven electrofusion techniques for studies of somatic cell genetics and of development, as well as for the generation of monoclonal antibodies. Animal Cell Electroporation and Electrofusion Protocols is an indispensable guide to animal cell electroporation for graduate and postdoctoral students, as well as laboratory directors in basic, applied, biomedical, biotechnological, and clinical research settings. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible success.

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