Capillary Electrophoresis of Proteins

This aims to be both a reference and a handbook. It is arranged by separation mode (zone electrophoresis, isoelectric focusing, and sieving) with discussions on separation principles, method of development and optimization. Practical details of buffer preparation, capillary selection and handling, and troubleshooting problem separations are emphasised. For readers who are new to the area of capillary electrophoresis, introductory chapters on the basic principles of capillary electrophoresis and CE instrumentation are included. The book explores methods of increasing sensitivity; details the use of buffer additives, surfactants, extreme pH and wall-coated capillaries; describes CE applications to enzyme assays, protein structure analysis and purification monitoring; highlights the effects of sample matrix, ampholyte composition and focusing mobilization conditions on isoelectric focusing analysis and reviews commercially available capillaries.

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