The large size and polyploid nature of the nuclear genome of bread wheat pose a great challenge for the understanding of complete genome information, genome sequencing and gene discovery.
This task could be simplified by fractionating the genome into small and defined parts. Here we propose to create two subgenomic BAC libraries specific for short and long arm of wheat chromosome 3D. Their creation will mark the completion of BAC resources for homeologous group 3, which has been selected for the pilot phase of the wheat genome sequencing project.
3D-specifc BAC resources developed in this project will be used to develop a molecular cytogenetic map and, in collaboration with Prof. J. Dvořák (UC, Davis), a physical contig map of 3D. They will also be used for targeted isolation of low-copy (potentially coding) DNA sequences, discovery of gene-rich regions on 3D, and for high-throughput mapping of expressed sequences to chromosome 3D using wheat GeneChip (collaboration with Prof. T. Close, UC, Davis).
The se advances will open avenues for comparative analysis of the wheat genome evolution and the analysis of interaction between the genomes of allopolyploid species. The results obtained and genomic resources generated, will allow the isolation and manipulation of important genes to improve tolerance of wheat to biotic and abiotic stresses and quality of plant products.
"www.ueb.cas.cz":[ http://www.ueb.cas.cz/Olomouc1/LMCC/projects.html]
Construction of cytogenetic map of the short arm of rye chromosome 1 (1RS) -
Rye is commonly used in wheat breeding and improvement as a source of resistance to abiotic stress, diseases and insects (23.5.2007)
New wheat variety aids fight against blight -
Researchers have created a new strain of wheat with an inbuilt resistance to devastating scab blight which infects wheat heads - reducing crop yields as well as market value and quality (26.3.2007)
Ancient genes used to produce salt-tolerant wheat -
In a recent set of papers published in the journal Plant Physiology researchers describe the two genes – known as Nax1 and Nax2 (4.2.2007)
Wheat's lost gene helps nutrition -
The gene occurs naturally in wheat, but has largely been silenced during the evolution of domestic varieties (30.11.2006)
Molecular characterization of Ph1 as a major chromosome pairing locus in polyploid wheat -
The foundation of western civilization owes much to the high fertility of bread wheat, which results from the stability of its polyploid genome (2.3.2006)
