Ph.D. position in pancreatic beta-cell research at Third Faculty of Medicine, Charles University, Prague, Czech Republic

Type of job: part-time

Project: Regulation of Beta-cell function and viability in relation to type 2 diabetes pathogenesis: molecular mechanisms

Dysfunction and apoptosis of pancreatic Beta-cells are key factors contributing to type 2 diabetes development. Pancreatic Beta-cells do not profit from the Western lifestyle, particularly low physical activity combined with an unhealthy diet (e.g., chronically increased intake of fatty acids, especially the saturated species). Some diabetes-associated pathological states (e.g., sleep apnea leading to chronic hypoxia in the pancreas) also negatively affect Beta-cells. The exact molecular mechanisms by which these and other diabetes-related factors affect Beta-cell function and viability are not yet revealed, despite clear medical implications in diabetes prevention and therapy. The aim of the project is to contribute to the elucidation and understanding of these mechanisms.

Methods of cell and molecular biology (Western blot, FACS, confocal microscopy, siRNA, CRISPR, etc.) will be employed to study the involvement of, e.g., miRNAs, various signaling pathways, autophagy and alternative cell death pathways (e.g., ferroptosis, necroptosis). As an experimental model, human and animal Beta-cell lines will be used. Alternatively, key results will be verified on isolated Langerhans islets. 

We are looking for a motivated and creative Ph.D. student interested in biomedical research and cell and molecular biology. Enthusiasm and an active approach to solving the topic are expected. The workplace is able to provide an increase in regular stipends. We offer work on a scientifically attractive topic under the guidance of experienced laboratory staff, support in career development, the opportunity to present results at foreign conferences, and implement foreign internships.

Contact:
RNDr. Vlasta Němcová, PhD.

E-mail:
vlasta.nemcova@lf3.cuni.cz

Updated: 31.10.2023