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FISH Technology

 

Authors: B. Rautenstraub and T. Liehr,
Publishing: Springer-Verlag
Published: 2002
Fluorescence in situ hybridization (FISH) is a powerful method to directly visualize the localization of genomic alterations in the nucleus. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells, FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue, simultaneous detection of apoptosis and expression of apoptosis-related genes, comparative genomic hybridization, special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH), FISH on insect cells and yeast, FISH-related techniques, such as PRINS and microarrays.

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