GM microbes created that can’t escape the lab

Critics of genetic engineering have long worried about the risk of modified organisms escaping into the environment. A biological-containment strategy described this week in Nature has the potential to put some of those fears to rest and to pave the way for greater use of engineered organisms in areas such as agriculture, medicine and environmental clean-up. 

Two US teams have produced genetically modified (GM) bacteria that depend on a protein building block — an amino acid — that does not occur in nature. The bacteria thrive in the laboratory, growing robustly as long as the unnatural amino acid is included in their diet. But several experiments involving 100 billion or more cells and lasting up to 20 days did not reveal a single microbe capable of surviving in the absence of the artificial supplement.

“Our strains, to the extent that we can test them, won’t escape,” says Dan Mandell, a synthetic biologist at Harvard Medical School in Boston, Massachusetts, and an author on one of the two studies describing the strategy.

The microbes also do not swap their engineered DNA with natural counterparts because they no longer speak life’s shared biochemical language. “Establishing safety and security from the get-go will really enable broad and open use of engineered organisms,” says Farren Isaacs, a synthetic biologist at Yale University in New Haven, Connecticut, who led the other study.

Biocontainment could provide added safety in the biological production of drugs or fuels, where microbes can be kept separate from their surroundings. But the modified bacteria could also permit controlled release into the human body or the environment. “Containment might no longer be of the physical kind,” says Tom Ellis, a synthetic biologist at Imperial College London who was not involved in the research.