Growing cells with 3D microgels
Date: 27.2.2014
The tool, which can be used to study cells in cost-efficient, three-dimensional microgels, may hold the key to personalized medicine applications in the future.
Stars, diamonds, circles: Rather than your average bowl of Lucky Charms, these are three-dimensional cell cultures generated by an exciting new digital microfluidics platform, the results of which have been published in Nature Communications this week by researchers at the University of Toronto.
"Everyone wants to do three-dimensional (3D) cell culture," explains Aaron Wheeler, Professor in Bioanalytical Chemistry at the Institute of Biomaterials & Biomedical Engineering (IBBME), the Department of Chemistry, and the Donnelly Centre for Cellular and Biomolecular Research (DCCBR) at the University of Toronto. "Cells grown in this manner share much more in common with living systems than the standard two-dimensional (2D) cell culture format," says Wheeler, corresponding author of the study.
More naturalistic, 3D cell cultures are a challenge to grow. "The reagents are expensive, the materials are inconvenient for automation, and 3D matrices break down upon repeated handling," explains Wheeler.
Team was able to address these difficulties by adapting a digital microfluidics platform first created in the Wheeler lab. Cells, caught up in a hydrogel material, are gently flowed across a small field that, on a screen, looks much like a tiny chessboard. The cells are strategically manipulated by a small electric field across a cutout shape on the top plate of the system, made from indium in oxide, and become fixed.
"When we grew kidney cells in these microgels, the cultures formed hollow sphere structures resembling primitive kidneys within four or five days," team member claims.
