Haematopoietic and Lymphoid Cell Culture

This reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact haematopoietic and lymphoid cells, and their maintenance as primary cultures. It provides technical information on the signals and mediators required for the differentiation and growth of these cells. Chapters discuss dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, hematopoietic differentiation of embryonal stem cells, and culturing of murine thymic explants.