Laboratory technician in Structural Virology

Type of job: full-time

Supervisor: Mgr. Pavel Plevka PhD

Research group description:

We use X-ray crystallography, cryo-electron microscopy, and molecular biology to study the life cycle of human viruses. We investigate macromolecular interactions associated with viruscell entry, genome replication, assembly, and maturation. Viruses are simple enough that we can aspire to understand their biology at a molecular level. Our efforts are directed towardsusing structural information for the development of anti-viral drugs and vaccines.

Job description:

We are looking for a highly motivated laboratory technician. Good interpersonal skills and basic knowledge of English are required. You will be involved in organizing the laboratory, purchasing chemicals and equipment, and performing routine laboratory tasks involving protein and virus production. You will be required to work with human picornaviruses in conditions of BSL2 containment. Previous experience with molecular biology and protein purification is desired. Basic knowledge in x-ray crystallography or electron microscopy is a big plus.

Experience with following techniques is required:

1. propagation of bacterial and mammalian cells
2. preparation of media for bacterial cultures and mammalian cell lines
3. basic knowledge of "laboratory chemistry" - preparation of common solutions, adjusting pH
4. molecular cloning - working with PCR, restriction enzymes, transformation of bacteria
5. computer literacy, MS-word, excel

Desired skills:

1. experience in working with viruses or phages, virus propagation and purification
2. experience with production, purification, and crystallization of recombinant proteins

 

Employment conditions:

Expected start: immediately

Candidates are encouraged to send CV and a short motivation letter including description of previous research and technical skills to Pavel Plevka (pavel.plevka@ceitec.muni.cz).

 

Pavel Plevka, PhD | CEITEC - Central European Institute of Technology | Masaryk University | Kamenice 5/A4/2.33, CZ-62500 Brno

Contact:
Pavel Plevka, PhD

E-mail:
pavel.plevka@ceitec.muni.cz

Updated: 28.7.2013