Neural Stem Cells: Methods and Protocols

Recognized experts describe the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. The techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. Protocols range from the simplest methods of isolation and characterization of neural cell properties to such sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.