Optimization of polysaccharide production by Alternaria alternata

Optimization of polysaccharide production by

Alternaria alternata

By

A.R.El-shamy and Nehad, E.A*

From

Department of Chemistry of Natural and Microbial Products, National Research Center, Dokki, Egypt

Abstract

Six strains of microorganisms were screened for Exopolysaccharide (EPS) production. Alternaria alternata was recognized to produce high levels of the EPS. The effects of environmental parameters on polysaccharide production by Alternaria alternata were investigated. Results showed the optimum temperature and initial pH for exopolysaccharide (EPS) production in shake flask cultures of Alternaria alternata were found to be 30°C and pH 3.0, respectively. Incubation period required for maximum production was 9days. Glucose was found to be the best carbon source to production of EPS, various concentrations of glucose were applied for the screening of the suitability of EPS production. 4% glucose was favorable to production of EPS. Yeast extract gave the highest amount of EPS by Alternaria alternata, its maximum concentration for EPS was determined.

Keywords: Alternaria alternata, EPS production, physiological conditions

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