Physiological Studies on Some Biologically Active Secondary Metabolites from Marine-Derived Fungus Penicillium brevicompactum

Physiological Studies on Some Biologically Active Secondary Metabolites from Marine-Derived Fungus Penicillium brevicompactum

By

Atalla, M. Mabrouk¹; Zeinab, H. Kheiralla²; Eman, R. Hamed¹; Amani, A. Youssry² and Abeer, A. Abd El Aty¹

From

¹ Chemistry of Natural and Microbial Products Dept. National Research Centre, Cairo, Egypt.

² Botany Dept. Faculty of Girls for Arts, Science and Education, Ain Shams University

Abstract

The fungal isolate Penicillium brevicompactum which isolated from the associated marine alga Pterocladia sp. in autumn season was able to produce 11 clear and active compounds, separated by the best solvent system dichloromethane: methanol (95:5 v/v). Compounds 4 and 9 were considered as antibacterial compounds, active against gram positive (B. subtillis) and gram negative (E. coli) bacteria. Malt extract broth medium with initial pH 4 when incubated at 28 ºC in an incubator shaker at 200 rpm for 12 days were the most favorable conditions for compound 4 production (19.87 mg/l). The suitable conditions for compound 9 production (121.13 mg/L) were potato carrot broth medium, initial pH 4, incubation temperature 26 ºC at 180 rpm after incubation period for10 days. Structural elucidation of the pure compounds suggested that compound 4 may be [Di(2-ethyl hexayl) phthalate], and compound 9 may be fungisterol or one of its isomers. Pure compounds were evaluated for cytotoxicity towards 6 different types of tumor cell lines performed in Cancer Biology Department, National Cancer Institute, Cairo University. The results revealed that, the maximum concentration of compound 4(100μg/mL) kills about 30% of lung cells. The maximum concentration of compound 9 (100 μg/mL) kills approximately 40% of the viable infected liver cells and also kills approximately 50% of the viable infected lung cells at concentration equal to 91.6 μg/mL. It can be concluded that compound 9 can be recommended as an anticancer compound.

Keywords: Marine fungi, Penicillium brevicompactum, Pharmaceuticals, Cytotoxicity, Anticancer compounds.

*Corresponding author: erhamed@yahoo.com

Citation: Mabrouk AM, Kheiralla ZH, Hamed ER, Youssry AA, Abd El Aty AA. (2011): Physiological Studies on Some Biologically Active Secondary Metabolites from Marine-Derived Fungus Penicillium brevicompactum. www.Gate2Biotech.com. 1 (1) 1-15

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