Researchers Alter Mosquito Genome With Goal of Controlling Disease

Virginia Tech researchers successfully used a gene disruption technique to change the eye color of a mosquito -- a critical step toward new genetic strategies aimed at disrupting the transmission of diseases such as dengue fever.

Zach Adelman and Kevin Myles, both associate professors of entomology in the College of Agriculture and Life Sciences and affiliated researchers with the Fralin Life Science Institute, study the transmission of vector-borne diseases and develop novel methods of control, based on genetics.

In a groundbreaking study published this week in the journal PLOS One, the scientists used a pair of engineered proteins to cut DNA in a site-specific manner to disrupt a targeted gene in the mosquito genome. Science magazine heralded these transcription activator-like effector nuclease proteins, known as TALENS, as a major scientific breakthrough in 2012, nicknaming them "genomic cruise missiles" for their ability to allow researchers to target specific locations with great efficiency. While TALENS have been previously used to edit the genomes of animal and human cell cultures, applying them to the mosquito genome is a new approach, according to Adelman.

To test the capability of TALENs to specifically edit the mosquito genome, the scientists designed a pair of TALENS to target a gene whose protein product is essential to the production of eye pigmentation in Aedes aegypti, a mosquito species known for its transmission of the viruses that cause dengue fever. Using the TALEN pair to edit the gene in the mosquito's germ cells early in development, they were able to change the eye color of a large percentage of the mosquitoes arising in the next generation from black to white.