Techniques for Molecular Biology

Content: Definition; Structure and Expression of a Eukaryote Gene Coding for an mRNA and a Protein; Parameters of Description of a Genome; Sequencing of Entire Genomes; Vectors and Cloning; Restriction Enzymes; Electrophoresis of Nucleic Acids; Description of a Plasmid and a Phagemid; Description of a Bacteriophage and a Cosmid; Description of a YAC and Other Large Vectors; Molecular Cloning; Genetic Transformation of Bacteria and Yeasts; Labelling of Nucleic Acids and Hybridization; DNA Labelling; Molecular Hybridization; In situ Hybridization of mRNA; DNA Libraries and Screening; Construction of a Genomic DNA Library; Construction of a cDNA Library; Screening of a Library; Differential Display: Subtractive Libraries, AFLP-cDNA; Differential Display RT-PCR: mRNA Screening; Differential Display SSH: Suppression Subtractive Hybridization; Differential Screening by RDA: Representational Difference Analysis; EST: Expressed Sequence Tags; DNA Microarrays: DNA Microchips, cDNA Filters; Characterization of a Gene; DNA Sequencing; PCR: Polymerase Chain Reaction; RACE: Rapid Amplification of cDNA Ends; Genome Walking by PCR; RT-PCR: Reverse Transcriptase PCR; In vitro Transcription; Determination of Transcription Initiation Site; Functional Analysis of Promoters; Gel Retardation; DNAse I Footprinting; Genetic Transformation of Euaryotes; Genetic Transformation of Plants by Agrobacterium tumefaciens; Direct Gene Transfer into Plant Protoplasts; Direct Gene Transfer by Biolistics; Genetic Transformation of Animal Cells; Cloning of Animals; Transient Expression; Analysis of Gene Function; Recombinant Proteins; Baculoviruses of Insects, Vectors of Expression of Foreign Genes; Double Hybrid System; Directed Mutagenesis; Mutation Complementation in Yeast; Knockout of Genes; Gene Tagging; RNA Interference; Polymorphism of a Genome; Molecular Markers; Genetic and Physical Maps; PFGE: Pulse Field Gel Electrophoresis; RFLP: Restriction Fragment Length Polymorphism; RAPD: Random Amplified Polymorphic DNA; AFLP: Amplified Fragment Length olymorphism; RETROMARKERS; SSCP: Single Strand Conformation Polymorphism; DGGE: Denaturing Gel Gradient Electrophoresis; SNP: Single Nucleotide Polymorphism; Simple Sequence Repeats (SSR): Microsatellites

Odkazy:

• http://www.scipub.net/biology/techniques-molecular-biology.html