| Solver: Zemánek Pavel, Vařecha Miroslav (Masaryk Univerzity), Liška Miroslav Brno (University of Technology), Lukášová Emilie (Institute of Biophysics AS CR), GA AV ČR IAA1065203, date: 01:01:2002 - 31:12:2006. The aim of the project is to use the methods and experimental systems of optical tweezers, optical scalpel and automated cytometer that were developed using the financial support of the previous scientific projects, for the study of the mechanical properties and arrangement of the interphase chromatin in the nuclei of living and fixed human cells. Theoretical and experimental experience of the interdisciplinary team of applicants will be used for: -fluorescence labelling of chromatin living cells, -laser induced fusion of living labelled cells, -topological analyses of the dye distribution inside living and fixed cells, insertion of dyed nanoparticle into the living cell nucleus, optical confinement of this probe and detection of the region which is not accesible for the probe, study of the mechanical properties of the chromatin using optical trapping of suitable probes. The knowledge obtained by these techniques will be used for development of new diagnostic methods. |
